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Tel : + 82 - 2 - 873 - 8168
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Background Technology

RNA-Guided ENdonucleases (RGEN), a new generation of engineered nuclease based on a bacterial CRISPR/Cas9 system, is innovative tools for the genome editing.
RGENs recognize a target sequence that is 19~20bps in lenth and is followed by sequence ending with two guanines(NGG) named PAM. This minimal requirement for target sequence recognition allows RGENs to be designed to edit any region of the genome (every 8bps, in theory). Specific cleavage of your target sequence allows efficient genome editing (knock-out / knock-in / locus deletion) in vitro and in vivo.
Our recent data is suggesting that the RGENs have a remarkably high efficiency and specificity in vitro. Additionally, our RGENs are able to support very robust production of gene-modified cell line, mouse and zebrafish.

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