Overview
Advantages of ToolGen's Genome Edited Cell Line Production Service
Customized Production
- Optimized sgRNA selection and cell line engineering through pre-consultation to meet the client’s specific objectives.
- Provides tailored strategies for cell lines that are difficult to edit, ensuring successful genome modification.
High-Efficiency Editing
- Mixed Cell Pool Service: Provides pools with up to 60% In/Del efficiency.
- Single Cell Clone Service: Enables selection of clones with 100% In/Del efficiency.
- Utilizes highly specific sgRNA and transfection with Cas9 protein (RNP) or mRNA to minimize off-target effects.
Various Applications
- Gene function research
- Customized disease model cell production
- Cell and gene therapy research and development
Workflow
Pre-Selected sgRNA
| Service | Item Descriptions | Period |
|---|---|---|
| Knock-Out Cell Line Service | Mixed Cell pool | 3-5 week |
| Single Cell Clone | 6-8 week | |
| Knock-In Cell Line Service | Mixed Cell pool | 8-10 week |
| Single Cell Clone | 11-13 week |
Novel sgRNA Screening
| Service | Item Descriptions | Period |
|---|---|---|
| Knock-Out Cell Line Service | Mixed Cell pool | 5-7 week |
| Single Cell Clone | 8-10 week | |
| Knock-In Cell Line Service | Mixed Cell pool | 10-12 week |
| Single Cell Clone | 13-15 week |
Optional QC Service
| Assay | Methods |
|---|---|
| Expression tests | RT-qPCR, WB, FACS |
| Chromosome analysis | Karyotyping |
| Off-target analysis | |